Title: 0150 - Oral Microbiome and Anthropometry Changes Following Caries Arrest Using Silver-Nitrate/Fluoride-Varnish
Hailey Taylor (Presenter)
University of California, San Francisco
Benjamin Chaffee, University of California San Francisco
Jeremy Horst, University of California, San Francisco
Marcus Duffin, Shoreview Dental
Steven Duffin, Shoreview Dental
Objectives: While studies have evaluated silver-fluoride combinations to arrest tooth decay, there is minimal information regarding the dental microbiome after intraoral silver use. Similarly, studies have evaluated the relationship between caries burden and anthropometric status, but the evidence is inconsistent. This study aimed to: 1. Evaluate changes in decayed tooth surfaces treated with SNFV over one year; 2. Assess associations between tooth decay and anthropometry, cross-sectionally and over time; 3. Compare the microflora of children treated and not treated with SNFV.
Methods: SNFV was applied to carious lesions in children ages 2-14 in rural Ghana (N=199). Tooth surfaces were re-assessed 12-months after application. Anthropometric status was calculated using WHO z-scores (height-for-age, BMI-for-age) at baseline and 12-months. Associations between z-scores and caries status were assessed using Spearman correlations. Stimulated saliva samples were collected from 38 children at 12-months for analyzing bacterial loads using rRNA gene sequencing.
Results: At baseline, 37% of children had ≥1 decayed tooth surface (mean: 5.3 surfaces). At 12-months, 69% of baseline decayed surfaces were arrested; 47% of children with baseline decay were free of active lesions. No meaningful association was found between baseline decay status and baseline anthropometry, nor change in height-for-age and decay reduction (all p>0.05). Correlation between change in BMI-for-age and decay reduction was weak but statistically significant (p=0.04). Bacterial ribosomal gene sequencing showed minimal differences in microbial abundances between children treated and not treated with SNFV, except children whose decay continued after treatment had significantly higher levels of S. mutans and Propionibacterium.
Conclusions: In this population, baseline tooth decay was not associated with baseline anthropometry, but reduction in decay was weakly associated with BMI-for-age increase. Treatment with SNFV reduced decay considerably and without differences in the natural microbiome 12-months after application, although children whose lesions completely arrested had significantly lower abundance of S. mutans.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE