Title: 1042 - A Multi-criterion Antimicrobial Assessment of a Chlorhexidine Mouthwash


Grace Haddad, The University of Manchester
Ruth Ledder, The University of Manchester
Prem Sreenivasan (Presenter)
Colgate-Palmolive, Co.

Andrew McBain, The University of Manchester


Objectives: Controlling the growth and metabolism of oral bacteria reduces the accumulation of dental plaque and associated metabolic products representing an important component of oral hygiene. This study utilized a number of experimental approaches to examine the antibacterial effects of a mouthwash formulated with 0.12% (v/v) chlorhexidine (marketed as Colgate PerioGard (PG)) in comparison to a fluoride control rinse (Colgate FluoriGard (FL).

Methods: The rapid antimicrobial effects of each mouthwash against Actinomyces viscosus were evaluated using a cell viability indicator dye which was also utilized to determine the minimum inhibitory concentration (MIC) of each mouthwash against Streptococcus mutans. The acidogenesis-mitigation effect for treatments was defined as the minimum acid neutralizing concentration (MNC) for S. mutans and evaluated by a previously described assay that utilized a pH indicator. A novel fluorescence-based sugar assay examined Minimum Sugar Metabolism Inhibitory Concentrations (MiSMIC) for S. mutans.

Results: PG demonstrated rapid inactivation of Actinomyces viscosus in comparison to FL with viability values of 0.02 for PG and 0.74 for FL. Additionally, the MIC values against S. mutans were <0.024% (v/v) for PG and >50% (v/v) for FL. Acidogenesis-mitigation effects represented by MNC values for PG and FL were <0.024% (v/v) and 25% (v/v) respectively. MiSMIC outcomes for PG were <0.024% (v/v) and 0.694% (v/v) for FL.

Conclusions: Together, these results demonstrate significant effects of the mouthwash formulated with 0.12% chlorhexidine for immediate antimicrobial effects on bacteria, inhibition of microbial acid production and sugar metabolism in comparison to a mouthwash formulated with fluoride. Results from this investigation provide additional rationale for the effectiveness of chlorhexidine for oral hygiene.

This abstract is based on research that was funded entirely or partially by an outside source:
Colgate Palmolive

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: This work was funded by Colgate Palmolive.

Sponsoring Group/Network