posterpresentation
Description

Title: 1424 - Effects of Radiopaque DAP on DPSC Proliferation and Mineralization

Authors:

Patrick McIntyre (Presenter)
Indiana University

Jennifer Wu, Indiana University
Rasika Kolte, Indiana University
Ghaeth Yassen, Indiana University
Angela Bruzzaniti, Indiana University

Abstract:

Objectives: A primary goal of endodontic regeneration is to eliminate as many pathogenic microorganisms as possible while allowing endogenous cells to regenerate the dentin-pulp complex. We investigated the proliferation and differentiation of human dental pulp stem cells (DPSCs) cultured with methylcellulose (MC) containing two radiopaque agents, 30% barium sulfate (BS) or 30% zirconium oxide (ZO), in combination with equal parts metronidazole and ciprofloxacin (DAP).

Methods: The experimental groups were MC+BS or MC+ZO alone or with 1-, 5- or 10-mg/mL DAP. Controls were culture media, MC only or calcium hydroxide [Ca(OH)2]. DPSCs were assayed for proliferation after 3 days. Additionally, DPSCs were cultured in osteogenic media and assayed for alkaline phosphatase (ALP) activity and mineralization, which are a measure of osteogenic differentiation. Experiments were replicated ≥3 times and data was analyzed using two-way ANOVA.

Results: The proliferation of DPSCs was similar between Ca(OH)2, MC+BS and MC+ZO controls, as well as all groups containing 1- or 5-mg/mL DAP, whereas DPSC proliferation was inhibited with 10-mg/mL DAP. Importantly, ALP activity was significantly increased in the MC+ZO and MC+BS controls, compared to MC only. Moreover, ALP activity was higher for MC+ZO compared to Ca(OH)2, suggesting an increase in DPSC differentiation by this radiopaque reagent. The MC+ZO+1-mg/mL DAP group also exhibited ALP activity similar to Ca(OH)2. Lastly, we found that mineral deposition by DPSCs was similar between the MC+ZO+1-mg/mL DAP, MC+BS+1-mg/mL DAP and the Ca(OH)2 control.

Conclusions: Together these results demonstrate that BS and ZO are not cytotoxic to DPSCs and that MC+ZO+1-mg/mL DAP is comparable to Ca(OH)2 in terms of DPSC proliferation, ALP activity and mineral deposition. Therefore, MC+ZO+1-mg/mL DAP may be a viable and superior alternative to Ca(OH)2 for endodontic applications as it not only can be viewed radiographically, but also promotes canal disinfection as well as the osteogenic differentiation of DPSCs to promote further root formation.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None

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