Title: 1433 - Cellular Response of Pulp Fibroblast to Single-multiple Photobiomodulation Applications


Maria Machado (Presenter)
University of Sao Paulo

Natalino Lourenço Neto, University of Sao Paulo
Luciana Vitor, University of Sao Paulo
Mariel Prado, University of Sao Paulo
Rodrigo Oliveira, University of Sao Paulo
Daniela Rios, University of São Paulo
Thiago Silva, University of São Paulo
Carlos Ferreira dos Santos, University of São Paulo
Thais Oliveira, Bauru School of Dentistry - University of São Paulo


Objectives: Objective: This study aimed to evaluate in vitro the effects of single or multiple photobiomodulation (PBM) applications on the viability and proliferation of pulp fibroblasts.

Methods: Methods: Pulp fibroblasts from human deciduous teeth (HdPF) were obtained from a biorepository, plated into 96-well plates, and irradiated according to the following groups: G1: 2.5 J/cm2 - 1X10 sec – 0.10 J; G2: 5 J/cm2; 1x 20 sec – 0.20 J; G3: 7.5 J/cm2 – 1x 30 sec – 0.30 J; G4: 2.5 J/cm2 - 2x 10 sec – 0.20J; G5: 2.5 J/cm2 – 3x 10 sec – 0.30 J; C+: not irradiated (DMEM 10% SFB); C-: not irradiated (DMEM 1% SFB). At 24h, 48h, and 72h after irradiation, cell viability and proliferation were assessed through MTT and Crystal Violet (CV) assays, respectively. The intergroup and intragroup analyses of the HdPF viability and proliferation were performed by two-way ANOVA followed by Tukey test, with level of significance set at 5%.

Results: Results: The intragroup comparison revealed statistically significant differences for G5 with increasing viability at 72 hours over 48 hours (p=0.027). The intergroup analysis showed a greater viability of the multiple photobiomodulation (PBM) applications (G5) over the single application (G3) at 72 hours. However, the proliferation was statistically significant greater for single PBM application over the multiple applications (G1>G5, G2>G3, and G2>G5).

Conclusions: Conclusion: The single application of 5 J/cm2 exhibited greater proliferation than the single application of 7.5 J/cm2 (G2>G3). Single and multiple PBM applications have different effects on pulp fibroblast from human deciduous tooth.

This abstract is based on research that was funded entirely or partially by an outside source:
Support Funding Agency/Grant Number: FAPESP grant # 2015/19362-5

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE

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