Title: 1434 - Nitric Oxide Releasing Nanomatrix Gel on Dental Pulp Stem Cells
Kyounga Cheon (Presenter)
University of Alabama at Birmingham
Catherine Porter, University of Alabama at Birmingham
David Cruz Walma, University of Alabama at Birmingham School of Dentistry
Jeremy Mao, Columbia University
Hui Wu, University of Alabama at Birmingham
Ho-Wook Jun, University of Alabama at Birmingham
Objectives: The study evaluated the effect of nitric oxide (NO) releasing biomimetic nanomatrix gel on cellular behavior of dental pulp stem cells (DPSCs).
Methods: The NO releasing nanomatrix gel was synthesized and self-assembled from peptide amphiphiles. DPSCs were purchased for the culture and treated with various volumes (25 µl, 50 µl, 100 µl, and 200 µl) of the self-assembled NO releasing biomimetic nanomatrix gel using Transwell®. Four experimental conditions were evaluated: a) Basal media without NO nanomatrix gel (B-N), b) basal media with NO nanomatrix gel (B+N), c) differentiation media without NO nanomatrix gel (I-N), and d) differentiation media with NO nanomatrix gel (I+N). Cellular behavior was measured using proliferation (CyQuant®), migration (3D Cell Culture Chips®), and differentiation (Alkaline Phosphatase and TaqMan quantitative reverse-transcriptase polymerase chain reaction) assays at three, seven, fourteen and twenty-one days.
Results: The NO releasing nanomatrix gel significantly promoted DPSC proliferation at day seven with 100 µl of NO gel (P < 0.05). DPSC migration toward the NO releasing nanomatrix gel was significantly increased at day fourteen and twenty-one (P < 0.05 Differentiation assay portrayed a significant effect on mRNA expression of dentin sialophosphoprotein and ALP increased by 2.6- and 3.1-fold at day seven and 4.5- and 5-fold at day twenty-one, respectively, in the condition of the I+N compared to B-N. Similarly, higher ALP activity was observed in the condition of the I+N compared to the B-N at day seven.
Conclusions: in vitro data imply that NO releasing nanomatrix gel promotes DPSC proliferation, migration and differentiation by providing an extracellular matrix-mimicking micro-environment. Biological function of the NO releasing nanomatrix gel will be further evaluated in vivo for its’ potential to serve as a pulp-dentin tissue revitalization therapy.
This abstract is based on research that was funded entirely or partially by an outside source:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None