Title: 0286 - Shear Bond Strength of Saliva Contaminated Dentin and Universal Adhesives


Ola Al Hatem (Presenter)
The University of Texas School of Dentistry at Houston

Anthony Rafaile, University of Texas at Austin
Joe Ontiveros, University of Texas at Houston
Magda Eldiwany, University of Texas School of Dentistry


Objectives: To compare universal adhesives bonded to human saliva contaminated dentin using light-cured and dual-cured resin cements.

Methods: Extracted human teeth were first sectioned in half using (Isomet; Buehler). Samples were mounted with cold-cure resin in cylinder molds, ground and polished to 320 grit finish (CarbiMet 2, Ecomet 6 Polisher; Buehler). A total of 144 samples were prepared for dentin, n=12. Two universal, 8th generation, adhesives, [(1) Scotchbond Universal; 3MESPE and (2) Adhese Universal VivaPen; Ivoclar] and one Total-Etch, 5th generation, adhesive (Control; OptiBond Solo Plus; Kerr) were used. Control group samples were etched with 35% phosphoric acid. Light cured (LC), (RelyX Veneer, A1; 3MESPE) and dual cured (DC) resin cements (Variolink Esthetic DC, Neutral; Ivoclar) were used. Two main groups were prepared, saliva contamination and no contamination. Cements were bonded using a shear bond testing jig (2.2 mm d, Ultradent) and light cured with a standard quartz-tungsten halogen (Optilux 501; SDS/Kerr). Specimens were stored in 100% humidity at 37°C for 24 hours. An Instron machine was used to load specimens and calculate shear bond strength in MPa. Data was analyzed using ANOVA and Tukey post hoc at 0.05 level of significance.

Results: A comparison of mean dentin bond strengths showed significant main effects for adhesive, p<0.001 and contamination, p<0.001. There was a statistically significant two-way interaction between adhesive and cement, p=0.04. ANOVA showed the 5th generation adhesive (OptiBond) resulted in higher mean dentin bond strengths compared to universal adhesives independent of contamination with saliva or cement type. Mean dentin bond strengths significantly dropped with saliva contamination for all adhesives using LC & DC resin cements, p<0.001.

Conclusions: Results suggest saliva contamination negatively effects shear bond strengths on dentin using universal adhesives. The universal adhesives tested showed lower mean dentin bond strengths compared to a Total Etch adhesive control.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE

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