Title: 0658 - Osteoinductive Potential of Silicon Nitride Ceramics Versus Mesenchymal Stem Cells


Tetsuya Adachi (Presenter)
Kyoto Prefectural University of Medicine

Satoshi Horiguchi, Kyoto Prefectural University of Medicine
Alfredo Rondinella, Kyoto Prefectural University of Medicine
Francesco Boschetto, Kyoto Prefectural University of Medicine
Elia Marin, Kyoto Prefectural University of Medicine
Toshiro Yamamoto, Kyoto Prefectural University of Medicine
Bryan McEntire, Amedica Corporation
Osam Mazda, Kyoto Prefectural University of Medicine
Narisato Kanamura, Kyoto Prefectural University of Medicine
Giuseppe Pezzotti, Kyoto Institute of Technology


Objectives: Silicon nitride (Si3N4) ceramic is employed in spinal implant devices because of its improved osteoconductivity and antibacterial properties. However, the osteoinductivity of Si3N4 is yet the object of investigations. This study analyzed the osteoinductive mechanisms of Si3N4 versus mesenchymal stem cells (MSCs) by using Raman spectroscopy and ELISA. The goal of this investigation was to determine whether the use of Si3N4 could be extended to dental implants.

Methods: In this study, the mouse MSC lines, KUSA-A1, was cultured in the Osteogenic Differentiation Medium on Si3N4 or Titanium substrates for 14 days. After 14 days, we observed the Calcified Matrix of KUSA-A1 with a Laser Raman microscope. In addition, the levels of Insulin-like growth factors (IGF-1) and Gla-Osteocalcin (Gla-OC) in the culture supernatant were measured by ELISA.

Results: We found that KUSA-A1 cultured on the Si3N4 substrates increased their production of IGF-1 and Gla-OC as compared to titanium substrates. Furthermore, culturing on the Si3N4 enhanced the calcification of KUSA-A1 more than titanium.

Conclusions: Si3N4 activates MSCs by producing silicic acid with chemical reactions. In addition, the roughness surface of Si3N4 synergistically accelerated osteoconductivity with physical stimulation. Si3N4 ceramics could rapidly obtain stable osteointegration. In conclusion, Si3N4 is extremely promising for dental implants and might contribute to increase the success rate of dental implants.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE

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