Title: 0952 - Effect of Arginine-calcium-carbonate and Adhesive-mode on Bond-strength to Dentin


Ana Acevedo (Presenter)
Universidad Central de Venezuela

Elizabeth Uoo, Rutgers University
Melisa Savascioglu, Rutgers University
Nicole Francisco, Stony Brook University
Danielle Wajngarten, Faculdade de Odontologia de Araraquara
Israel Kleinberg, Stony Brook University
Ana Botta, Stony Brook University


Objectives: To assess, in vitro, the effect of 8% arginine and calcium carbonate (A) and two different adhesive modes on microtensile bond strength of a universal adhesive agent to intact (I) and caries-affected dentin (C).

Methods: Ten intact third molars and ten decayed third molars were sectioned with a low-speed diamond saw to obtain two middle dentin specimens per tooth. A universal adhesive agent (Prime & Bond Elect Universal, Dentsply) was applied to intact and caries-affected dentin in two different modes: Etch-and-Rinse (ER- phosphoric acid application for 15s) and Self-Etch (SE- no phosphoric acid application). 8% arginine and calcium carbonate (Sensitive Pro-Relief toothpaste, Colgate) was applied to intact and caries-affected dentin for 1min. Specimens were randomly assigned into 8 groups (n=5): SEI; ERI; ASEI; AERI; SEC; ERC; ASEC; and AERC. All specimens were restored with the universal adhesive agent and TPH Spectra universal composite (Low viscosity, A2 shade, Dentsply). Specimens were sectioned to obtain sticks with a cross-section of 0.8±0.2mm2. All sticks were tested 24h after the restorative procedure at a crosshead speed of 1mm/min. The data were evaluated statistically by the non-parametric three-way ANOVA followed by the pairwise comparisons’ test (α=0.05).

Results: The interaction among “dentin substrate”, “adhesive mode” and “arginine-calcium-carbonate” did not present significant statistical difference (p=0.345). However, the self-etch mode reduced the bond strength to caries-affected dentin (ERC=23.77MPa; SEC=8.59MPa; p<0.001). The application of arginine and calcium carbonate decreased significantly the bond strength to dentin (No Arginine=23.77MPa; Arginine=17.02MPa; p=0.021).

Conclusions: Etch-and-Rinse technique enhanced the bond strength to caries-affected dentin. The bond strength to intact dentin was not affected by the adhesive mode. 8% arginine and calcium carbonate reduced the bond strength regardless dentin substrate and adhesive mode.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None

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