Title: 0963 - The Effects of Epigallocatechin-gallate on Dental Adhesives and MMP Inhibition
Saleh Alhijji (Presenter)
L. Jack Windsor, Indiana University
Objectives: Matrix metalloproteinase (MMPs) have been shown to play a substantial role in dentine’s collagen matrix breakdown, which may contribute to the potential failure of dental restorations over time. Therefore, modifications in the current dental adhesive system are necessary that would inhibit the unwanted activities of the MMPs. Epigallocatechin-3-gallate (EGCG) was selected as a MMP inhibitor to be incorporated within the dental adhesives. The main objective of this study was to investigate the potential changes in the chemical and mechanical properties of modified-dental adhesives by adding different concentrations of EGCG and evaluate their ability to inhibit MMP activity, thus aiming to improve the integrity and biostability of the resin-dentin interface.
Methods: Disk-shaped (n=6 per group) specimens of dental adhesive (3M, Adper™ Scotchbond™ Multi-Purpose Plus) were prepared containing different concentrations of EGCG (0% as a control, 0.01%, 0.005%, 0.0025%, and 0.00125%). Gelatin zymography was used to assess each concentration of EGCG inhibitory effects on human MMP-1 (PeproTech, Rocky Hill, NJ) proteolytic activity. The degree of conversion (DC) by Fourier-transform infrared spectroscopy (FTIR) was utilized to relativity compare the degree of polymerization among the groups. Knoop-microhardness test was also performed as an indication for any variations in the mechanical properties with each EGCG concentration.
Results: Analysis of variance (ANOVA) of the MMP-1 activity by zymography shows a significant dose-dependent inhibition of the EGCG groups compared to the control group (p<0.005). No significant statistical differences were found among the groups in the Degree of conversion (DC) test (p=0.185), as well as Knoop-microhardness tests (p= 0.252). However, a positive correlation was found between DC and Knoop-microhardness using Pearson correlation coefficient (p<0.001).
Conclusions: The incorporation of EGCG within dental adhesives could potentially expand the life-span of dental restorations in the clinic by enhancing the biostability and integrity of resin-dentin interface. Further investigation on the EGCG release over time at the resin-dentin interface is needed to ensure its safety and its ability to inhibit the MMPs and protect the resin-dentin interface.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE