Title: 1727 - Methylsufonylmethane (MSM) Induces Differentiation Of Human Exfoliated Deciduous Teeth (SHED) Stem Cells Into Bone Forming Cells
Hanan Aljohani (Presenter)
University of Maryland Dental School
Meenakshi Chellaiah, University of Maryland Dental School
Objectives: Our approach is to induce jaw bone augmentation in the presence of suitable stem cell line and a factor which has multiple potential.
Our objectives are as follows:
1. Identify a stem cell line which has the ability to differentiate into osteoblast.
2. Identify the effect of MSM on osteogenic differentiation as an alternative to the commonly used osteogenic medium and pricy bone morphogenic proteins (BMPs)
3. Identify the combinatorial effects of stem cell, MSM and bone particles on osteogenesis
Methods: Different stem cell lines were used to identify a stem cell which demonstrates the characteristics of osteoblasts. The expression of osteogenic markers was examined using immunoblotting and real-time PCR analysis for protein and mRNA expression, respectively. Alizarin red and calcein blue mineralization assays were used to assess osteogenic differentiation.
Results: Our key findings are as follows:
1) When screening several cell lines, we recognized stem cells from human exfoliated deciduous teeth (SHED) is appropriate for the process of bone repair in jaws. SHED cells, a) express osteogenic markers (Ostrix, osteopontin, Runx2, and osteocalcin) at mRNA and protein levels as indicators of osteoblast differentiation; b) have the ability to express alkaline phosphates and produce mineralization nodules in the presence of osteogenic medium.
2) MSM indeed induces osteogenic property of SHED cells in the ABSENCE of bone morphogenic proteins (BMPs) and osteogenic medium. Osteogenic markers indicated above have been measured. MSM effects on the osteogenic potential of SHED cells is either equal or more than the effects mediated by osteogenic medium
3) Moreover, combination of MSM and bone particles induces osteogenic and mineralization potential of SHED cells to a significant level
Conclusions: Results from these studies offer new strategies for possible use of SHED cells and MSM in improving bone healing process. MSM is a potential cost-effective osteoinductive material as opposed to BMPs.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE