Title: 1097 - Comparison of Enamel Fluoride Uptake of Three Different Fluoride Varnishes
Maria Al Ismail (Presenter)
Henry M. Goldman School of Dental Medicine, Boston University
Yuwei Fan, Henry M. Goldman School of Dental Medicine, Boston University
Dan Nathanson, Henry M. Goldman School of Dental Medicine, Boston University
Objectives: The objective is to compare the anti-caries efficacy of F varnishes by studying their ability to deliver fluoride.
Methods: Three 5% NaF varnishes were used in this study, ProFluorid (VOCO), Vanish (3M ESPE), StarBright (Nanova Biomaterials) and artificial saliva solution as a control. Twenty-four extracted intact permanent teeth were randomly divided into 4 groups (n=6) for three fluoride varnishes and a control group. Each group was tested under two protocols (n=3 for each protocol). In Protocol A, artificial lesions were created by immersing the sound teeth in Coca Cola for 20 mins at 37 °C for demineralization (DM). Then fluoridation step was performed by applying a thin layer of around 5 mg of fluoride varnish on enamel surface at room temperature (FV1). All specimens for all four groups were submerged in 30 mL of artificial saliva for 24 hr at 37°C. Following the treatment period, extra fluoride varnish was removed from each specimen using chloroform moistened cotton swab and all teeth were then cleaned with deionized water for 10 seconds. The fluoridation cycle was repeated once more (FV2). For subgroups under Protocol B, tooth specimens with sound enamel were treated with two cycles of fluoridation (FV1 and FV2), then exposed to Coca Cola for 20 mins demineralization (DM). Fluoride content of each tooth was analyzed under Scanning Electron Microscopy/Energy Dispersive Spectrometry (SEM/EDS) at baseline, after each fluoride varnish application, and demineralization treatment. Weight percentage of fluoride was recorded for each specimen and the differences within treatment groups are analyzed by JMP Pro 13 using ANOVA.
Results: Fluoride contents of tested groups are shown in Table 1.
Conclusions: This preliminary study concludes that application of NaF varnish twice can significantly increase the fluoride content in enamel in both remineralization and protection cases. The high mean value and deviation of fluoride content after application of ProFluorid was probably due to local formation of calcium fluoride deposits.
Image(s):Download Image 1
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE