Title: Oral Bacteria, Yeast, Cortisol Response in Young Caries-Free Children
Dorota Kopycka-Kedzierawski (Presenter)
University of Rochester
Thomas OConnor, University of Rochester
Kathy Scott-Anne, University of Rochester
Patricia Ragusa, University of Rochester
Changyang Feng, University of Rochester
Robert Quivey, University of Rochester
Gene Watson, University of Rochester
Marija Cvetanovska, University of Rochester
Ronald Billings, University of Rochester
Objectives: Objectives: To assess the carriage of Streptococcus mutans, Lactobacilli, Candida species and salivary cortisol response in young, clinically caries-free children.
Methods: Methods: Whole saliva was collected from 115 clinically caries-free children 1 to 3 years of age; this is the preliminary analysis of a 2-year longitudinal study of 200 children at risk for Early Childhood Caries (ECC). Saliva samples were evaluated for Streptococcus mutans, Lactobacilli, and Candida species [using Mitis Salivarius agar and bacitracin (MSB), Rogosa, and CHROMagar, respectively]. Salivary cortisol samples were analyzed using ELISA.
Results: Results: At baseline, 8% of the children did not carry any Streptococcus mutans; almost 20% of the children carried Lactobacilli and 24% of the children carried Candida species, either Candida albicans or non albicans Candida; non-albicans Candida included: Candida tropicalis, Candida glabrata and Candida krusei. Preliminary analyses indicated that oral bacteria and yeast carriage were significantly associated with the child’s primary caregiver’s education indicators at baseline (OR=.02, p=.03 for Lactobacilli, OR=.07, p=.04 for Candida species), and at 6 months (OR=.04, p=.03 for Candida species). At 6 months, children who carried Candida species were more likely to respond to a stressful situation as indicated by child salivary cortisol, a marker of stress physiology that may be linked with risk for ECC (OR=3.5). Children who experienced ECC at 6 months were more likely to be Hispanic (OR=2.7), carry Streptococcus mutans (OR=2) and, their primary caregiver being worried (OR=1.4) and depressed (OR=1.7).
Conclusions: Conclusions: A majority of the young, clinically caries-free children carried either Streptococcus mutans, Lactobacilli or Candida species (including non-albicans Candida) in the oral cavity. Positive response to stress in children at socio-economic disadvantage and primary caregiver psychosocial traits opens a new pathway for assessing caries risk in this population. Supported by R01 DE024985