Title: Influence of Toothpaste Fluoride Concentration and Remineralization Time on Erosive Tooth Wear
Sarah Buedel (Presenter)
Indiana University School of Dentistry
Frank Lippert, Indiana University School of Dentistry
George Eckert, Indiana University School of Medicine
Anderson Hara, Indiana University School of Dentistry
Objectives: Remineralization of eroded dental surfaces can decrease their susceptibility to toothbrushing abrasion; however, the influence of toothpaste fluoride concentration on this protection is not known. This in-vitro study investigated how toothbrushing with toothpaste slurries (0ppm F, 1100ppm NaF, 1100ppm SnF2, 5000ppm NaF) simulated after different remineralization times (0, 30, and 60 min) impacted the development of erosive tooth wear lesions.
Methods: Enamel and root dentin specimens were prepared from bovine incisors (n=8) and submitted to a cycling protocol including erosion, remineralization at the test times, and toothbrushing, for five days. Dental surface loss (SL, in micrometers) was determined by optical profilometry. Data were analyzed using mixed-model ANOVA and Fisher’s PLSD tests (alpha=0.05).
Results: For toothpaste comparison, 1100ppm SnF2 had significantly less SL than 0ppm, 1100ppm NaF, and 5000ppm NaF (all p<0.05), regardless of substrate or remineralization time. 0ppm did not differ from 1100ppm NaF or 5000ppm NaF (p>0.05), except when 5000ppm NaF was used on enamel after 60min remineralization (p<0.05). 1100ppm NaF did not differ from 5000ppm NaF, except when used on dentin substrate after 30min of remineralization, or on enamel substrate after 60min (p<0.05). When comparing remineralization times, for 0ppm, regardless of substrate, and for 1100ppm SnF2 used on enamel, 0min remineralization had significantly higher SL than 30min (p<0.05) and 60min (p<0.05); with no difference between 30 and 60min (p>0.05). For 1100ppm SnF2 on dentin, remineralization time had no effect (p>0.05). For 1100ppm NaF and 5000ppm NaF, regardless of substrate, no clear linear trend was noted.
Conclusions: Overall, the toothpaste containing 1100ppm SnF2 demonstrated the greatest potential for protection against toothbrushing abrasion on both enamel and dentin, which was enhanced by remineralization time for enamel, but not for dentin.