Title: Farnesol Anti-biofilm Activity Against Candida albicans Reference and Mutant Strains
Adriana Carreiro, UFRN
Sarah Guedes, UFC
Beatriz Panariello, Araraquara Dental School- São Paulo State University (FOAr- UNESP)
Paula Silveira, UFC
Malvin Janal, NYU College of Denstistry
Simone Duarte (Presenter)
Indiana University School of Dentistry
Objectives: To determine the anti-biofilm role of farnesol on Candida albicans by comparing its effect on a reference strain with its effect on two mutant strains that were defective in the ability to form hyphae and/or biofilm.
Methods: tt-farnesol solutions with a concentration of 12.5 mM were prepared. Biofilms (n=6) of the strains C. albicans SN425 (reference strain), C. albicans CJN2330 (Δ/Δ tec1) and C. albicans CJN2302 (Δ/Δ efg1) were treated during biofilm formation with tt-farnesol or a vehicle control (1 min) twice daily (9 am and 3 pm) until the third day of the experiment. Biofilms were also treated with 0.2% chlorhexidine-CHX (1 min) as a positive control and 0.89% NaCl (1 min) as a negative control. After treatments, biofilms were evaluated by dry weight (biomass-μg) and colony forming units (CFU/mL).
Results: ANOVA showed an interaction between strain and treatment factors (p < 0.001). Farnesol showed inhibitory effect on C. albicans by reducing the rank of CFU/mL of all the strains when compared to the negative control [CFU/mL -rank(SD)- Farnesol: C. albicans SN425 24.167(4.335); C. albicans CJN2330 35.000(11.278); C. albicans CJN2302 33.833(9.621); Control: C. albicans SN425 48.167(11.071); C. albicans CJN2330 67.500(4.506); C. albicans CJN2302 47.833(8.565)], and the biomass of the mutant strain C. albicans CJN2330 [dry weight µg(SD) - Farnesol: 313(124); Control: 532(237)].
Conclusions: Twice daily treatment with tt-farnesol in a concentration of 12.5 mM exhibited anti-biofilm activity against different strains of C. albicans.