Title: Immunohistochemical Expression of RUNX2 in Condylar Hyperplasia
Adam Sorenson (Presenter)
Seth Butcher, Marquette University
Andrea Doetzer, Pontifícia Universidade Católica do Paraná
Carla Loreto, Catania University
Rosalia Leonardi, Catania University
Morteza Rasoulianboroujeni, Marquette University School of Dentistry
Lobat Tayebi, Marquette University School of Dentistry
Luis Eduardo Almeida, Marquette University
Objectives: This study will examine the association of runt-related transcription factor 2 (RUNX2) expression and condylar hyperplasia (CH). CH is defined as excessive growth of one mandibular condyle, leading to facial asymmetry, cross-bite, and open bite. The etiology of CH is unknown, but has been associated with localized trauma and infection. RUNX2 is a protein associated with osteoblastic differentiation and has been previously proposed as an associated factor in CH. This study will directly examine the association between RUNX2 expression and CH.
Methods: Immunohistochemical analysis of 17 human condyle samples from healthy and affected patients will be performed. 3-3’ Diaminobenzidine (DAB) immunohistochemistry will be used to statistically compare CH condyles with their normal counterparts.
Results: Immunohistochemical staining has shown a difference in the expression of RUNX2 between the control group and the CH group. The experimental group has demonstrated more intense staining with the DAB chromogen than the control group.
Conclusions: We conclude that RUNX2 expression may be correlated with the development of CH. This study may further clarify the etiology and pathogenesis of CH, leading to improved diagnostic and therapeutic options for those affected.