Description
Presentation Blocks: 03-22-2018 - Thursday - 03:45 PM - 05:00 PM

Title: Differential Expression of Glycosylation Related Genes During Wound Healing

Authors:

Veronica Haywood (Presenter)
University of Illinois at Chicago

Lin Chen, University of Illinois at Chicago
Karen Colley, University of Illinois at Chicago
Luisa DiPietro, University of Illinois at Chicago

Abstract:

Objectives: The tongue exhibits refined angiogenesis and a reduced inflammatory response to wounding when compared to the skin. Recently our lab discovered that the expression of sialic acid related genes and glycoproteins bearing terminal sialic acid monosaccharides mirrors the differential inflammatory response to wounding observed in the skin and tongue. Having a better understanding of the mechanisms that contribute to the tongue’s advantageous inflammatory response may suggest targeted pathways that can improve healing in post-surgical craniofacial wounds and pathological mucosal tissues. The objective of this study was to elucidate the role of glycosylation during wound healing by identifying whether overall glycosylation related gene expression was differentially regulated during tongue and skin wound healing.

Methods: Glycosylation related gene expression was analyzed from microarray data (GEO-GSE23006) examining the tongue and skin at baseline and in 1mm excisional wounds (harvested at 6hr, 12hr, 24hr, and days 3, 5, 7, and 10 post-wounding, n=3 per time point). Differential expression of glycosylation related genes were evaluated by Two-way ANOVA with Bonferroni correction of relevant comparisons.

Results: Of 183 genes examined, 119 were significantly regulated during tongue or skin wound healing, and 74 of the significantly regulated genes were differentially expressed between the tongue and skin during the inflammatory phase (6-24h) of wound healing (Fold Change [FC]>1.5, p<0.05). Among these genes, Lyg2 (an antibacterial enzyme) was downregulated (1.8 fold) while multiple glycosphingolipid related genes and Chi3l1 (which may increase microbial attachment) was upregulated (1.5-14 fold) during skin wound healing (p<0.05).

Conclusions: This data provides evidence that additional glycosylation related genes may modulate the differential inflammatory response observed between skin and tongue wounds. While future studies are required to determine causation, this data proposes a role for changes in the skin microbiome as a potential cause for the enhanced inflammation observed in skin versus oral mucosal wound healing.

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