Title: Effect of Piezotome on Bone Regeneration in the Absence and Presence of Bisphosphonate
Fahad Aljamal (Presenter)
Alaa alqutub, Boston University
Serge Dibart, Boston University
Erdjan Salih, Boston University
Objectives: To determine the effects of the Piezotome on bone regeneration in the presence and absence of Bisphosphonate.
Methods: live bone surgical defects on neonatal mouse calvarial bone organ cultures were used and categorized in 2 groups. Group (A) was treated with bisphosphonate 6 days prior to defects while other group (B) treated with bisphosphonate at the same time of defects. A set of calvaria in each group were treated with either Piezotme and Bur mounted on high speed handpiece. The controls were corresponding sets of calvaria with no piezotome treatment. The impacts of the piezotome and bur on bone were evaluated by quantitative analysis of calcium release and uptake, bone repair/healing and bone resorption. In addition, non-critical defects were performed on a set of calvarial bone and followed to assess defect closure in groups treated with and without Bisphosphonate. Control group with defect only, Piezotme injury 4 mm away from the defect and Bur injury 4 mm away from the defect.
Results: In surgical defects the repair/healing was significantly enhanced when the piezoelectric knife was used when compared with the use of traditional carbide bur for defect generation. In group (a), Piezo groups showed less bone resorption in resorption system and more bone formation in formation system. In contrast, Piezo groups in group (b) shows the opposite with more resorption and less formation in resorption and formation systems respectively. In defect closure, all groups with Bisphosphonate treatment showed initial defect healing in the first few days only. In Contrast, defects without Bisphosphonate showed more cellular migration and defect healing. Piezotome treated group was the highest to promote cellular migration and defect closure.
Conclusions: This study shows that the effect of the piezotome is much stronger on bone biology in both resorption and formation systems and bone healing compared to carbide bur. Bones treated with bisphosphonate shows reduced bone resorption but apparent more bone formation, indicating that individuals subjected to bisphosphonate therapy may have longer duration for orthodontic movement.