Title: Antibiotic Elution Efficacy of a Novel Titanium Implant Polymer Coating
Amer Tiba, Naval Medical Research Unit San Antonio
Monica Johnson, NAMRU-SA
Luis Martinez, NAMRU- SA
John Simecek (Presenter)
Objectives: Surfaces of titanium implants used to correct cranial defects are susceptible to bacterial colonization and biofilm formation. Tailored implant surface coatings offer the potential to target specific bacteria commonly found in biofilms causing implant failure. The objective of this study was to assess the elution effectiveness of a novel light-cured single component polymer resin implant coating with antibiotic eluting capability to inhibit Methicillin-resistant Staphylococcus aureus (MRSA).
Methods: Methicillin-resistant Staphylococcus aureus was plated on a 100 mm petri dish containing nutrient agar to confluency. Titanium coupons were prepared with the polymer resin loaded with antimicrobials vancomycin or berberine at concentrations of 0.3mg/mL and 5.0 mg/ml, respectively. As controls, uncoated titanium coupons, titanium coupons coated with unloaded polymer resin, and whatman papers with or without a 5.0 mg/ml concentration of vancomycin were placed in the middle of the confluent cultures and incubated for 24hrs at 37°C. Zones of inhibition were measured on the control and test plates, and reported as means and standard deviations for three independent experiments.
Results: The data indicate that the coupons with the polymer resin loaded with vancomycin at a concentration of 5.0 mg/ml had a zone of inhibition of 31.5±1.3 mm. No zones of inhibition were observed for the uncoated titanium coupons, titanium coupons coated with unloaded polymer resin, or berberine loaded polymer resin. The whatman paper vancomycin loaded controls had a zone of inhibition of 34.75±0.35 mm at a concentration of 5.0 mg/ml.
Conclusions: The novel polymer resin has the capability of eluting loaded vancomycin at concentrations capable of inhibiting the growth of MRSA. Berberine loaded coupons were not observed to have antimicrobial activity. Further research on the effectiveness of the novel polymer resin to inhibit biofilm progression is currently being assessed.