Title: Alcohol-free Antimicrobial Mouthrinses Inhibit Human Supragingival Plaque Regrowth and Glycolysis
Shantia Kazemi, University of Illinois at Chicago
Wei Li, University of Illinois at Chicago
Christine Wu (Presenter)
University of Illinois at Chicago
Objectives: Antimicrobial alcohol-containing mouthrinses with cetylpyridinium chloride (CPC) or combination of essential oils (EO) have demonstrated efficacy in controlling human dental plaque. Alcohol-free mouthrinses are available for consumers who have dry mouth, oral hypersensitivity, or those who wish to avoid use of alcohol. We hypothesize that alcohol-free mouthrinses are as effective as alcohol-containing rinses in inhibiting human plaque regrowth and acid production. This study evaluated the short-term effects on human supragingival plaque regrowth and glycolysis after rinsing with two alcohol-free antimicrobial EO and CPC mouthrinses. Comparison was made between these rinses and an alcohol-containing EO mouthrinse and a water placebo control.
Methods: Eleven healthy adults (18 to 64 years) participated in this randomized controlled crossover study. The three commercially marketed mouthrinses used were an alcohol-containing EO rinse (A-EO), an alcohol-free essential oils mouthrinse (AF-EO) and an alcohol-free CPC-containing mouthrinse (AF-CPC). Water was used as the negative control. Overnight fasting supragingival plaque from buccal and lingual surfaces in the left maxillary and mandibular quadrants of a participant were collected (Baseline untreated plaque). The participants then rinsed with one of the three mouthrinses or water for 30 sec, their right maxillary and mandibular plaque was collected after 30 min. All plaque samples were further assessed for acid production and regrowth after 2 and 4hr incubation at 37°C. There was a three-day washout between visits.
Results: All three mouthrinses significantly inhibited plaque acid production (2hr, ΔpH: 0.28-0.84) and regrowth (4hr, EO, 67.4%; AF-EO, 66.2%; AF-CPC, 88.2%) compared to water rinse (p<0.05). The AF-CPC mouthrinse demonstrated superior efficacy in reducing acid production after rinsing, compared to both the AF-EO and alcohol-containing EO mouthrinses (p≤0.001). There was no significant difference in efficacy between AF-EO and alcohol-containing EO mouthrinses.
Conclusions: Selected alcohol-free mouthrinses are effective alternatives to alcohol-containing rinses in suppressing short-term plaque regrowth and acid production.