Presentation Blocks: 03-23-2018 - Friday - 11:00 AM - 12:15 PM

Title: Bacterial Extrusion From Infected Root Canals During Masticatory Function


Ashraf Fouad, University of North Carolina
Taiseer Sulaiman, University of North Carolina
Ali Altitinchi (Presenter)
University of North Carolina


Objectives: Several studies have documented significant associations between endodontic infections and cardiovascular disease. The purpose of this study is to use a contemporary ex-vivo model to determine the extent to which bacteria may migrate from infected root canals with dynamic loading.

Methods: Streptococcus intermedius was selected to determine bacterial migration under static and dynamic loading conditions. Extracted maxillary anterior teeth were accessed, had their canals prepared to standardized size and inoculated anaerobically with standardized concentrations of bacteria for 21 days. They were then divided into two groups (n=5): dynamic and static loading. Controls with no bacterial inoculation or loading were included. Teeth in the dynamic group were subject to loading using a chewing simulator (CS-4.2, SD Mechatronik, Germany). After simulated chewing of one-year duration, aliquots of the respective periapical transport media were collected, and quantitative analysis was assessed by counting the colony forming units (CFUs) using serial dilution.

Results: No growth was observed in the control group. All specimens in the dynamic group had bacterial penetration, whereas 2 of five specimens in the static group had no bacterial penetration. Significantly more bacterial CFUs were present in the dynamic group compared with the static group (p=0.002).

Conclusions: Results of this study demonstrated that mastication may aid in the migration of S. intermedius beyond the apical foramen. These findings warrant performing clinical studies in future, to determine whether these results can be replicated in vivo.


Poster Session

11:00 am–12:15 pm Mar 23 (US - Eastern)

CC, Hall B/C